Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: DLGAP status between GC and NG tissues from TCGA database. The results showed that no significant differences were discovered in the expression of (A) DLGAP1, (B) DLGAP2 and (C) DLGAP3. However, (D) DLGAP4 and (E) DLGAP5 were significantly upregulated in GC tissues when compared with NG tissues. DLGAP, discs large-associated protein; GC, gastric cancer; NG, normal gastric.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: DLGAP status between matched GC and APC tissues from The Cancer Genome Atlas database. The results showed that consistent results for (A) DLGAP4 and (B) DLGAP5 were obtained when the 34 matched pairs of GC and APC tissues were compared. GC, gastric cancer; APC, adjacent para-cancer; DLGAP, discs large-associated protein.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: DLGAP status in GC from the Gene Expression Omnibus database and clinical samples. The results showed no significant difference in the expression of (A) DLGAP1 and a significant difference in the expression of (B) DLGAP5 between GC and normal gastric tissues. Due to the lack of expression data for DLGAP2 and DLGAP3, the present study was unable to evaluate its expression and perform statistical analysis. In addition, as only two samples were available for (C) DLGAP4, a P-value could not be generated. (D) Reverse transcription-quantitative polymerase chain reaction analysis of 19 pairs of clinical samples revealed a similar trend. DLGAP, discs large-associated protein; GC, gastric cancer.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Expressing, Generated, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: Expression of DLGAP4 between GC and APC tissues using imunohistochemistry. The results demonstrated that DLGAP4 was overexpressed in GC tissues when compared with APC tissues. (A) DLGAP4; (B) APC; (C) DLGAP4 at higher magnification; (D) APC at higher magnification. GC, gastric cancer; APC, adjacent para-cancer; DLGAP, discs large-associated protein.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: Prognostic significance of DLGAPs in gastric cancer, assessed by OncoLnc. The results showed that out of all the DLGAP family members, only the overexpression of DLGAP4 was correlated with shorter overall survival. (A) DLPGAP1; (B) DLGAP2; (C) DLGAP3; (D) DLGAP4; (E) DLGAP5. DLGAP, discs large-associated protein.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Over Expression

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: Prognostic significance of DLGAPs in GC, assessed by Kaplan-Meier plotter. The results revealed that the expression of all DLGAP family members except DLGAP3 was correlated with the prognosis of patients with GC. (A) DLPGAP1; (B) DLGAP2; (C) DLGAP3; (D) DLGAP4; (E) DLGAP5. DLGAP, discs large-associated protein; GC, gastric cancer.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: Alterations and coexpression network of DLGAP4. (A) DLGAP4 alterations, as determined by cBioPortal included (from high to low) missense mutations, amplification, mRNA upregulation and truncating mutations. (B) The coexpression network of DLGAP4 from Cytoscape; the top 100 genes were selected and included in the network. DLGAP4, discs large associated protein 4.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Amplification

Journal: Molecular Medicine Reports

Article Title: Examination of the expression and prognostic significance of DLGAPs in gastric cancer using the TCGA database and bioinformatic analysis

doi: 10.3892/mmr.2018.9574

Figure Lengend Snippet: FunRich analysis. The possible biological processes (A) and biological pathways (B) of DLGAP4 as determined by FunRich. FunRich, Functional Enrichment tool.

Article Snippet: At the same time, tissues were also fixed in 4% formaldehyde at room temperature for 48 h in preparation for immunohistochemistry (IHC) ( ) with rabbit anti-DLGAP4 primary antibody (BIOSS, Beijing, China; cat. no. AE080301) in PBS (1:500) overnight at 4°C.

Techniques: Functional Assay